Fundamental Approaches to Screen Abnormalities in Drosophila free ebook download.

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Fundamental Approaches to Screen Abnormalities in Drosophila free ebook download

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Physics

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Author(s):	Monalisa Mishra

Date:	2019-11-16	Format:	pdf	Language:	English	ISBN/ASIN:	1493997556
Pages:	300	OCR:		Quality:		ISBN13:
Uploader:	maveriks	Upload Date:	12/17/2019 7:01:10 PM

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This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided. The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining  determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays  and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophilas eye and head via paraffin section  measurement of reactive oxygen species from various tissues via FACS  and the CRISPER technique for gene editing and analysis of micro RNA mutations.

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2653737	Book	https://nitroflare.com/view/8A7C5B7B5A32...		9.37	maveriks

Tags: Abnormalities Approaches Drosophila Fundamental Screen

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