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Description of  Cell-free Protein Synthesis: Methods and Protocols

From the Back Cover Complex biomolecules such as proteins cannot simply be synthesized by standard chemical reactions. They need to be assembled making use of the biological mechanisms that generate them in the living cell. Recreating the biosynthesis of proteins in a controlled cell-free laboratory system is no trivial task, yet brings enormous benefits in terms of product cost and purity that are particularly relevant for biotechnological applications. With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, this practical handbook covers the latest advances in the field both on the analytical and the preparative scale. Following an introduction, the book goes on to deal with the constructive approach for cell-free translation, recombinant human proteins, and cell-free expression and production. It also covers novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering, as well as gene cloning and expression in molecular colonies. The step-by-step protocols and practical examples given for each method represent practical advice for beginners and experts alike.